Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal ImagingJanlo M. Robil 1,2, Cedric David T. Cortez 1, Chloe Margalaux R. Villafuerte 1, Edgar Daniel C. Dela Peña 1, Derreck O. De Leon 1, Roselle Joy R. Rioja 1, Paula McSteen 2
1Department of Biology, School of Science and Engineering, Ateneo de Manila University, 2Division of Biological Sciences, Interdisciplinary Plant Group, and Missouri Maize Center, University of Missouri
Maize leaf primordia are deeply ensheathed and rolled, making them difficult to study. Here, we present methods for preparing transverse sections and unrolled whole mounts of maize leaf primordia for fluorescence and confocal imaging.
