Ateneo de Manila University

2 ARTICLES PUBLISHED IN JoVE

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Biology

Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging
Janlo M. Robil 1,2, Cedric David T. Cortez 1, Chloe Margalaux R. Villafuerte 1, Edgar Daniel C. Dela Peña 1, Derreck O. De Leon 1, Roselle Joy R. Rioja 1, Paula McSteen 2
1Department of Biology, School of Science and Engineering, Ateneo de Manila University, 2Division of Biological Sciences, Interdisciplinary Plant Group, and Missouri Maize Center, University of Missouri

Maize leaf primordia are deeply ensheathed and rolled, making them difficult to study. Here, we present methods for preparing transverse sections and unrolled whole mounts of maize leaf primordia for fluorescence and confocal imaging.

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Biology

A Concoction Pipeline for Generating Molecular Operational Taxonomic Units (MOTUs) Among Riparian and Aquatic Beetles
Emmanuel D. Delocado 1,2, Voltaire Rafael H. Banzon, Jr. 1, Enrico Gerard S. Sanchez 1,3
1Ateneo Biodiversity Research Laboratory, Department of Biology, School of Science and Engineering, Higher Education Cluster, Ateneo de Manila University, 2Ateneo Institute of Sustainability, Ateneo de Manila University, 3Ateneo School of Medicine and Public Health, Higher Education Cluster, Ateneo de Manila University

The protocol presented here aims to guide the users on the computer-based processing of cytochrome c oxidase subunit I (COI) gene sequences generated from beetles, such that the species clustering hypothesis called molecular operational taxonomic units (MOTUs) can be generated from DNA.