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Hunan Normal University

4 ARTICLES PUBLISHED IN JoVE

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Engineering

Generation of Size-controlled Poly (ethylene Glycol) Diacrylate Droplets via Semi-3-Dimensional Flow Focusing Microfluidic Devices
Yan Wu 1, Xiang Qian 1, Shengli Mi 1, Min Zhang 1, Shuqing Sun 1, Xiaohao Wang 1,2
1Graduate School at Shenzhen, Tsinghua University, 2The State Key Laboratory of Precision Measurement Technology and Instruments, Tsinghua University

Here, we present a protocol to illustrate the fabrication processes and verifying experiments of a semi-three-dimensional (semi-3D) flow-focusing microfluidic chip for droplet formation.

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Bioengineering

Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices
Gordon D. Hoople *1, Andrew Richards *2, Yan Wu 2, Albert P. Pisano 3, Kun Zhang 2
1Department of Engineering, University of San Diego, 2Department of Bioengineering, University of California, San Diego, 3Department of Mechanical and Aerospace Engineering, University of California, San Diego

Gel-seq enables researchers to simultaneously prepare libraries for both DNA- and RNA-seq at negligible added cost starting from 100 - 1000 cells using a simple hydrogel device. This paper presents a detailed approach for the fabrication of the device as well as the biological protocol to generate paired libraries.

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Biology

A Bacterial Oral Feeding Assay with Antibiotic-Treated Mosquitoes
Xinyi Liu 1,2, Si Wu 1,2, Wenqian Li 1,2, Meihong Zhang 1,2, Yan Wu 1,2, Ning Zhou 3, Pa Wu 1,2
1Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, College of Life Science, Hunan Normal University, 2State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Provincial Key Laboratory for Microbial Molecular Biology, College of Life Science, Hunan Normal University, 3Department of Infectious Diseases, the Second Xiangya Hospital, Central South University

This article presents a protocol to investigate the effect of individual mosquito gut bacteria, including isolation and identification of mosquito midgut cultivable microbes, antibiotic depletion of mosquito gut bacteria, and reintroduce one specific bacteria species.

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Medicine

2D-HPLC-MS Technology Combined with Molecular Network for the Identification of Components in Tibetan Medicine Aconitum pendulum
Jing Ma *1, Hanyu Lu *1, Jia Liu 1, Ting Wang 1, Xing Fu 2, Xinmei Xu 2, Yi Zhang 1, Jing Zhang 1, Xiaolong Xie 1, Yingzhuang Chen 3,4, Jinsong Su 1
1Ethnic Medicine Academic Heritage Innovation Research Center, Meishan Hospital, Chengdu University of Traditional Chinese Medicine, 2State Key Laboratory of Southwestern Chinese Medicine Resources, Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, 3Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, 4Key Laboratory of Phytochemical R&D of Hunan Province, Hunan Normal University

This study utilizes two-dimensional high-performance liquid chromatography-mass spectrometry (2D-HPLC-MS) technology in conjunction with molecular networking to unravel the intricate chemical composition of the Tibetan medicinal plant Aconitum pendulum Busch (APB). The article provides a detailed protocol for the systematic exploration and identification of complex chemical components of herbal medicines.

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