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Hunan Normal University

4 ARTICLES PUBLISHED IN JoVE

Engineering

Generation of Size-controlled Poly (ethylene Glycol) Diacrylate Droplets via Semi-3-Dimensional Flow Focusing Microfluidic Devices

Yan Wu¹, Xiang Qian¹, Shengli Mi¹, Min Zhang¹, Shuqing Sun¹, Xiaohao Wang^1,2

¹Graduate School at Shenzhen, Tsinghua University, ²The State Key Laboratory of Precision Measurement Technology and Instruments, Tsinghua University

Here, we present a protocol to illustrate the fabrication processes and verifying experiments of a semi-three-dimensional (semi-3D) flow-focusing microfluidic chip for droplet formation.

Bioengineering

Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices

Gordon D. Hoople^*1, Andrew Richards^*2, Yan Wu², Albert P. Pisano³, Kun Zhang²

¹Department of Engineering, University of San Diego, ²Department of Bioengineering, University of California, San Diego, ³Department of Mechanical and Aerospace Engineering, University of California, San Diego

Gel-seq enables researchers to simultaneously prepare libraries for both DNA- and RNA-seq at negligible added cost starting from 100 - 1000 cells using a simple hydrogel device. This paper presents a detailed approach for the fabrication of the device as well as the biological protocol to generate paired libraries.

Biology

A Bacterial Oral Feeding Assay with Antibiotic-Treated Mosquitoes

Xinyi Liu^1,2, Si Wu^1,2, Wenqian Li^1,2, Meihong Zhang^1,2, Yan Wu^1,2, Ning Zhou³, Pa Wu^1,2

¹Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, College of Life Science, Hunan Normal University, ²State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Provincial Key Laboratory for Microbial Molecular Biology, College of Life Science, Hunan Normal University, ³Department of Infectious Diseases, the Second Xiangya Hospital, Central South University

This article presents a protocol to investigate the effect of individual mosquito gut bacteria, including isolation and identification of mosquito midgut cultivable microbes, antibiotic depletion of mosquito gut bacteria, and reintroduce one specific bacteria species.

Medicine

2D-HPLC-MS Technology Combined with Molecular Network for the Identification of Components in Tibetan Medicine Aconitum pendulum

Jing Ma^*1, Hanyu Lu^*1, Jia Liu¹, Ting Wang¹, Xing Fu², Xinmei Xu², Yi Zhang¹, Jing Zhang¹, Xiaolong Xie¹, Yingzhuang Chen^3,4, Jinsong Su¹

¹Ethnic Medicine Academic Heritage Innovation Research Center, Meishan Hospital, Chengdu University of Traditional Chinese Medicine, ²State Key Laboratory of Southwestern Chinese Medicine Resources, Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, ³Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, ⁴Key Laboratory of Phytochemical R&D of Hunan Province, Hunan Normal University

This study utilizes two-dimensional high-performance liquid chromatography-mass spectrometry (2D-HPLC-MS) technology in conjunction with molecular networking to unravel the intricate chemical composition of the Tibetan medicinal plant Aconitum pendulum Busch (APB). The article provides a detailed protocol for the systematic exploration and identification of complex chemical components of herbal medicines.