University Medical Centre Groningen, University of Groningen

1 ARTICLES PUBLISHED IN JoVE

Bioengineering

Continuous High-resolution Microscopic Observation of Replicative Aging in Budding Yeast

Daphne H. E. W. Huberts¹, Georges E. Janssens², Sung Sik Lee³, Ima Avalos Vizcarra⁴, Matthias Heinemann^1,5

¹Molecular Systems Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, ²Department for the Biology of Ageing, European Research Institute for the Biology of Ageing, University Medical Centre Groningen, University of Groningen, ³Institute of Biochemistry, ETH Zurich, ⁴Department of Health Sciences and Technology, ETH Zurich, ⁵Institute of Molecular Systems Biology, ETH Zurich

We describe here the operation of a microfluidic device that allows continuous and high-resolution microscopic imaging of single budding yeast cells during their complete replicative and/or chronological lifespan.