National Research Council of Italy
2 ARTICLES PUBLISHED IN JoVE
Bioengineering
Measurement of Tension Release During Laser Induced Axon Lesion to Evaluate Axonal Adhesion to the Substrate at Piconewton and Millisecond Resolution
Massimo Vassalli 1, Michele Basso 2, Francesco Difato 3
1Institute of Biophysics, National Research Council of Italy, 2Dipartimento di Sistemi e Informatica, Università di Firenze, 3Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia
We measured the tension release in an axon that was partially lesioned with a laser dissector by simultaneous force spectroscopy measurement performed on an optically-trapped probe adhered to the membrane of the axon. The developed experimental protocol evaluates the axon adhesion to the culture substrate.
Bioengineering
Experimental and Data Analysis Workflow for Soft Matter Nanoindentation
Giuseppe Ciccone 1, Mariana Azevedo Gonzalez Oliva 1, Nelda Antonovaite 2, Ines Lüchtefeld 3, Manuel Salmeron-Sanchez 1, Massimo Vassalli 1
1Centre for the Cellular Microenvironment, James Watt School of Engineering, University of Glasgow, 2Optics 11 life, 3Laboratory of Biosensors and Bioelectronics, ETH Zürich
The protocol presents a complete workflow for soft material nanoindentation experiments, including hydrogels and cells. First, the experimental steps to acquire force spectroscopy data are detailed; then, the analysis of such data is detailed through a newly developed open-source Python software, which is free to download from GitHub.
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