Ruhr University
2 ARTICLES PUBLISHED IN JoVE
Biology
Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
Louise E. Bird 1, Heather Rada 1, Anil Verma 1, Raphael Gasper 2,3, James Birch 4, Matthew Jennions 4, Jan Lӧwe 3, Isabel Moraes 4, Raymond J. Owens 1
1Oxford Protein Production Facility, Research Complex at Harwell, 2Protein Crystallography Group, Ruhr University, 3MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, 4Membrane Protein Laboratory, Diamond Light Source
A streamlined approach to screening for the expression of recombinant membrane proteins in Escherichia coli based on fusion to green fluorescent protein is presented.
Medicine
Imaging Metals in Brain Tissue by Laser Ablation - Inductively Coupled Plasma - Mass Spectrometry (LA-ICP-MS)
Dominic J. Hare 1,2, Kai Kysenius 3, Bence Paul 4, Beate Knauer 5,6, Robert W. Hutchinson 7, Ciaran O'Connor 7, Fred Fryer 8, Tom P. Hennessey 8, Ashley I. Bush 2, Peter J. Crouch 3, Philip A. Doble 1
1Elemental Bio-imaging Facility, University of Technology Sydney, 2Florey Institute of Neuroscience and Mental Health, The University of Melbourne, 3Department of Pathology, The University of Melbourne, 4School of Earth Sciences, The University of Melbourne, 5Research School, Ruhr University, 6Department of Physiology, Monash University, 7ESI Ltd., Bozeman, 8Agilent Technologies, Mulgrave
Quantitatively mapping metals in tissue by laser ablation - inductively coupled plasma - mass spectrometry (LA-ICP-MS) is a sensitive analytical technique that can provide new insight into how metals participate in normal function and disease processes. Here, we describe a protocol for quantitatively imaging metals in thin sections of mouse neurological tissue.
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