Accedi

Ruhr University

2 ARTICLES PUBLISHED IN JoVE

Biology

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

Louise E. Bird¹, Heather Rada¹, Anil Verma¹, Raphael Gasper^2,3, James Birch⁴, Matthew Jennions⁴, Jan Lӧwe³, Isabel Moraes⁴, Raymond J. Owens¹

¹Oxford Protein Production Facility, Research Complex at Harwell, ²Protein Crystallography Group, Ruhr University, ³MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, ⁴Membrane Protein Laboratory, Diamond Light Source

A streamlined approach to screening for the expression of recombinant membrane proteins in Escherichia coli based on fusion to green fluorescent protein is presented.

Medicine

Imaging Metals in Brain Tissue by Laser Ablation - Inductively Coupled Plasma - Mass Spectrometry (LA-ICP-MS)

Dominic J. Hare^1,2, Kai Kysenius³, Bence Paul⁴, Beate Knauer^5,6, Robert W. Hutchinson⁷, Ciaran O'Connor⁷, Fred Fryer⁸, Tom P. Hennessey⁸, Ashley I. Bush², Peter J. Crouch³, Philip A. Doble¹

¹Elemental Bio-imaging Facility, University of Technology Sydney, ²Florey Institute of Neuroscience and Mental Health, The University of Melbourne, ³Department of Pathology, The University of Melbourne, ⁴School of Earth Sciences, The University of Melbourne, ⁵Research School, Ruhr University, ⁶Department of Physiology, Monash University, ⁷ESI Ltd., Bozeman, ⁸Agilent Technologies, Mulgrave

Quantitatively mapping metals in tissue by laser ablation - inductively coupled plasma - mass spectrometry (LA-ICP-MS) is a sensitive analytical technique that can provide new insight into how metals participate in normal function and disease processes. Here, we describe a protocol for quantitatively imaging metals in thin sections of mouse neurological tissue.