Methods for Performing Crosses in Setaria viridis, a New Model System for the GrassesHui Jiang 1, Hugues Barbier 2, Thomas Brutnell 1
1Donald Danforth Plant Science Center, 2Boyce Thompson Institute
We have developed a methodology for performing crosses in Setaria viridis (S. viridis). The method involves pruning the panicle prior to a hot water treatment to kill viable pollen. Crosses are performed following a well-controlled growth regime and typically result in the recovery of 1 to 7 cross-pollinated seed/s per panicle.
