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Boyce Thompson Institute

1 ARTICLES PUBLISHED IN JoVE

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Methods for Performing Crosses in Setaria viridis, a New Model System for the Grasses

Hui Jiang¹, Hugues Barbier², Thomas Brutnell¹

¹Donald Danforth Plant Science Center, ²Boyce Thompson Institute

We have developed a methodology for performing crosses in Setaria viridis (S. viridis). The method involves pruning the panicle prior to a hot water treatment to kill viable pollen. Crosses are performed following a well-controlled growth regime and typically result in the recovery of 1 to 7 cross-pollinated seed/s per panicle.