Universidad Icesi

2 ARTICLES PUBLISHED IN JoVE

Biology

Detecting SARS-CoV-2 Virus by Reverse Transcription-Loop-Mediated Isothermal Amplification

Steven A. David-Jimenez¹, Paola A. Caicedo¹, Maria F. Villegas-Torres^1,2, Natalia Campillo-Pedroza^1,3

¹Facultad de Ingeniería, Diseño y Ciencias Aplicadas, Universidad Icesi, ²Centro de Investigaciones Microbiológicas (CIMIC), Department of Biological Sciences, Universidad de los Andes, ³BioDx: Diagnóstico y Soluciones Biotecnológicas S.A.S, Universidad Icesi

Here we provide a complete protocol to standardize and implement the method for detecting the SARS-CoV-2 virus in human samples by reverse transcription loop-mediated isothermal amplification (RT-LAMP). This method, done within 60 min, could be adapted to any laboratory or point-of-care at a low cost and using inexpensive equipment.

Medicine

Preservation of Porcine Biomodels for the Study of Comparative Human Anatomy

Liliana Valladares-Torres¹, Flavio Edgar Cerón-Betancourth¹, Jose Guillermo Betancourt-Villalobos², Angelica Benavidez-Jaramillo^1,3, Sergio Andrés Montenegro-Herrera¹

¹Departamento de Ciencias Básicas Médicas, Facultad de Ciencias de la Salud, Universidad Icesi, ²Departamento de Salud Pública y Medicina Comunitaria, Facultad de Ciencias de la Salud, Universidad Icesi, ³Translational Research Laboratory in Critical Care Medicine (TransLab-CCM), Universidad Icesi

This article presents a protocol for preserving porcine biomodels. The proposed method involves utilizing a modified Chilean solution with a reduced formalin concentration. The preservation process consists of administering the solution through both repletion and immersion of the specimen, followed by deformolization and tissue softening using glycerin.