Universidad Icesi

Here we provide a complete protocol to standardize and implement the method for detecting the SARS-CoV-2 virus in human samples by reverse transcription loop-mediated isothermal amplification (RT-LAMP). This method, done within 60 min, could be adapted to any laboratory or point-of-care at a low cost and using inexpensive equipment.

This article presents a protocol for preserving porcine biomodels. The proposed method involves utilizing a modified Chilean solution with a reduced formalin concentration. The preservation process consists of administering the solution through both repletion and immersion of the specimen, followed by deformolization and tissue softening using glycerin.