Emmanuel College

2 ARTICLES PUBLISHED IN JoVE

Upper Extremity Transplantation in Non-Human Primates: An Orthotopic Model for Translational Research.

David A Leonard^1,2,3, Harrison Powell¹, Alexander Albritton¹, Kumaran Shanmugarajah^1,2, Melissa Mastroianni^1,2, Sarah Lofgren¹, James Winter¹, Josef M Kurtz^1,4, Curtis L Cetrulo, Jr^1,2

¹Transplantation Biology Research Center, Massachusetts General Hospital, Harvard Medical School, ²Division of Plastic and Reconstructive Surgery, Massachusetts General Hospital, Harvard Medical School, ³Department of Plastic & Reconstructive Surgery Research, University of Manchester, ⁴Department of Biology, Emmanuel College

Vascularized composite allotransplantation (VCA) offers unparalleled restoration of function and form following devastating musculoskeletal and soft tissue injury. Surgical techniques have developed rapidly, but immunological and functional outcomes require further study. We described a rigorous non-human primate model of orthotopic upper extremity transplantation for pre-clinical studies immediately preceding clinical translation.

Biology

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage

Emily K. Matozel¹, Nathaniel Dale¹, Allen C. Price²

¹Department of Biology, Emmanuel College, ²Department of Chemistry and Physics, Emmanuel College

A highly parallel method for measuring the site-specific cleavage of DNA at the single molecule level is described. This protocol demonstrates the technique using the restriction endonuclease NdeI. The method can easily be modified to study any process that results in site-specific DNA cleavage.