Vascularized composite allotransplantation (VCA) offers unparalleled restoration of function and form following devastating musculoskeletal and soft tissue injury. Surgical techniques have developed rapidly, but immunological and functional outcomes require further study. We described a rigorous non-human primate model of orthotopic upper extremity transplantation for pre-clinical studies immediately preceding clinical translation.
A highly parallel method for measuring the site-specific cleavage of DNA at the single molecule level is described. This protocol demonstrates the technique using the restriction endonuclease NdeI. The method can easily be modified to study any process that results in site-specific DNA cleavage.
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