Instituto Politecnico Nacional

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary

Carlos Eduardo Espinosa-de la Garza^*1, Rodolfo Daniel Salazar-Flores^*1, Néstor Octavio Pérez¹, Luis Francisco Flores-Ortiz¹, Emilio Medina-Rivero¹

¹Unidad de Investigación y Desarrollo, Probiomed S.A. de C.V.

Here, we present a comprehensive capillary zone electrophoresis protocol for the assessment of intrinsic physicochemical heterogeneity of monoclonal antibodies as a quality attribute.

Medicine

Determination of the Relative Potency of an Anti-TNF Monoclonal Antibody (mAb) by Neutralizing TNF Using an In Vitro Bioanalytical Method

Lilia Tierrablanca-Sánchez¹, Víctor Pérez Medina Martínez¹, Nancy D. Ramírez Ibañez¹, Néstor O. Pérez Ramírez¹, Francisco Luis Flores Ortiz¹, Emilio Medina-Rivero²

¹Research and Development, Probiomed, ²Bioprocess Research and Development Unit, Instituto Politecnico Nacional

A protocol for the determination of the relative anti-apoptotic activity of an anti-TNFα mAb using a neutralization mechanism with WEHI 164 cells is presented here. This protocol is useful for comparing the neutralization strength of different molecules with the same biological functionality.