Instituto Politecnico Nacional
2 ARTICLES PUBLISHED IN JoVE
Biochemistry
Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary
Carlos Eduardo Espinosa-de la Garza *1, Rodolfo Daniel Salazar-Flores *1, Néstor Octavio Pérez 1, Luis Francisco Flores-Ortiz 1, Emilio Medina-Rivero 1
1Unidad de Investigación y Desarrollo, Probiomed S.A. de C.V.
Here, we present a comprehensive capillary zone electrophoresis protocol for the assessment of intrinsic physicochemical heterogeneity of monoclonal antibodies as a quality attribute.
Medicine
Determination of the Relative Potency of an Anti-TNF Monoclonal Antibody (mAb) by Neutralizing TNF Using an In Vitro Bioanalytical Method
Lilia Tierrablanca-Sánchez 1, Víctor Pérez Medina Martínez 1, Nancy D. Ramírez Ibañez 1, Néstor O. Pérez Ramírez 1, Francisco Luis Flores Ortiz 1, Emilio Medina-Rivero 2
1Research and Development, Probiomed, 2Bioprocess Research and Development Unit, Instituto Politecnico Nacional
A protocol for the determination of the relative anti-apoptotic activity of an anti-TNFα mAb using a neutralization mechanism with WEHI 164 cells is presented here. This protocol is useful for comparing the neutralization strength of different molecules with the same biological functionality.
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