Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus ProteinsN. Bishara Marzook *1, Dean J. Procter *1, Helena Lynn 1, Yui Yamamoto 2, Jacquelyn Horsington 3, Timothy P. Newsome 1
1School of Molecular Bioscience, University of Sydney, 2Department of Medicine, Center for Vascular Research, 3Asia Pacific Centre for Animal Health, Faculty of Veterinary Science, University of Melbourne
A rapid and modular protocol for the generation of recombinant vaccinia viruses expressing fluorescently tagged proteins simultaneously using the method of transient dominant selection is described here.
