qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy TissueMorgan H. McCoy 1, Kristin Post 2, Joyashree D. Sen 2, Hsim Y. Chang 2, Zijin Zhao 1, Rong Fan 1, Shaoxiong Chen 1, Diane Leland 1, Liang Cheng 1, Jingmei Lin 1
1Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, 2Department of Pathology and Laboratory Medicine, Indiana University Health
This protocol describes qPCR detection of cytomegalovirus in formalin-fixed, paraffin-embedded biopsy tissue, which is rapid, sensitive, specific, and useful for interpreting equivocal hematoxylin and eosin or immunohistochemical staining patterns.
