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Indiana University Health

1 ARTICLES PUBLISHED IN JoVE

Biology

qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue

Morgan H. McCoy¹, Kristin Post², Joyashree D. Sen², Hsim Y. Chang², Zijin Zhao¹, Rong Fan¹, Shaoxiong Chen¹, Diane Leland¹, Liang Cheng¹, Jingmei Lin¹

¹Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, ²Department of Pathology and Laboratory Medicine, Indiana University Health

This protocol describes qPCR detection of cytomegalovirus in formalin-fixed, paraffin-embedded biopsy tissue, which is rapid, sensitive, specific, and useful for interpreting equivocal hematoxylin and eosin or immunohistochemical staining patterns.