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VA Puget Sound Health Care System

4 ARTICLES PUBLISHED IN JoVE

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Behavior

Assessment of Murine Exercise Endurance Without the Use of a Shock Grid: An Alternative to Forced Exercise
Jennifer D. Conner 1,2, Tami Wolden-Hanson 1, LeBris S. Quinn 1,3,4
1Research Service, VA Puget Sound Health Care System, 2Seattle Institute for Biomedical and Clinical Research, 3Division of Gerontology and Geriatric Medicine, Department of Medicine, University of Washington, 4Geriatric Research, Education, and Clinical Center, VA Puget Sound Health Care System

A method to assess exercise endurance in laboratory mice without the use of a shock grid is demonstrated. This method is a humane refinement that can decrease the confounding effects of stress on experimental parameters.

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Medicine

Acclimation Prior to an Intraperitoneal Insulin Tolerance Test to Mitigate Stress-Induced Hyperglycemia in Conscious Mice
Rebecca L. Hull 1,2, Daryl J. Hackney 1, Elizabeth L. Giering 1,2, Sakeneh Zraika 1,2
1Division of Metabolism, Endocrinology and Nutrition, VA Puget Sound Health Care System, 2Department of Medicine, University of Washington

Stress-induced elevations in glucose levels can confound interpretation of data derived from a conscious intraperitoneal insulin tolerance test in mice. In this article, we describe a method to acclimate the mice to handling, injections and blood sampling prior to performing the insulin tolerance test in order to limit stress-induced hyperglycemia.

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Medicine

Laser Doppler Perfusion Imaging in the Mouse Hindlimb
Gale L. Tang 1,2, Kevin J. Kim 2
1Division of Vascular Surgery, University of Washington, 2VA Puget Sound Health Care System

Here, we present a protocol that demonstrates the technique and necessary controls for Laser Doppler perfusion imaging to measure blood flow in the mouse hindlimb.

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Neuroscience

Modeling Highly Repetitive Low-level Blast Exposure in Mice
Adam Crabtree *1, Cory McEvoy *1,2, Peter Muench 1, Rebecca A. Ivory 3,4,5, Josh Rodriguez 5, Mohamed Omer 5, Trinity Charles 5, James S. Meabon 5,6
1United States Army Special Operations Command, 2CU Anschutz Center for COMBAT Research, Department of Emergency Medicine, University of Colorado School of Medicine, 3University of Washington School of Nursing, 4University of Delaware School of Nursing, 5Veterans Affairs Northwest Mental Illness Research, Education, and Clinical Center (MIRECC), VA Puget Sound Health Care System, 6Department of Psychiatry and Behavioral Sciences, University of Washington School of Medicine

Presented here are methods for producing repeated low-intensity blast exposures using mice.

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