Korea Atomic Energy Research Institute

3 ARTICLES PUBLISHED IN JoVE

Chemistry

An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a ¹²⁵I-labeled Azide Prosthetic Group

Jongho Jeon^1,2, Ha Eun Shim¹, Sajid Mushtaq^1,2, Mi Hee Choi¹, Sang Hyun Park^1,2, Dae Seong Choi¹, Beom-Su Jang^1,2

¹Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, ²Department of Radiation Biotechnology and Applied Radioisotope Science, University of Science and Technology

A detailed procedure for the synthesis of a ¹²⁵I-labeled azide and the radiolabeling of dibenzocyclooctyne (DBCO)-group-conjugated, 13-nm-sized gold nanoparticles using a copper-free click reaction is described.

Engineering

An Efficient Method for Selective Desalination of Radioactive Iodine Anions by Using Gold Nanoparticles-Embedded Membrane Filter

Ha Eun Shim^*1,2, Sajid Mushtaq^*1,3, Jongho Jeon^1,3

¹Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, ²Department of Chemistry, Kyungpook National University, ³Department of Radiation Biotechnology and Applied Radioisotope Science, University of Science and Technology

An efficient method for the rapid and ion-selective desalination of radioactive iodine in several aqueous solutions is described by using gold nanoparticles-immobilized cellulose acetate membrane filters.

Biology

Quantification, Viability Assessment, and Visualization Strategies for Acinetobacter Biofilms

Joo-Sung Kim^1,2, Jihoon Lim³

¹Korea Food Research Institute, ²Department of Food Biotechnology, Korea University of Science and Technology, ³Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute

This protocol describes the preparation of the inoculum, the biofilm quantification on microtiter plates using crystal violet dye, the viable count in biofilms, and the visualization of biofilms of Acinetobacter.