Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCRAlessandra Sottini 1, Federico Serana 1, Diego Bertoli 1, Marco Chiarini 1, Monica Valotti 1, Marion Vaglio Tessitore 1, Luisa Imberti 1
1CREA, Diagnostics Department, Spedali Civili di Brescia
Here, we describe a method for simultaneous quantification of T-cell receptor excision circles (TRECs) and K-deleting recombination excision circles (KRECs). The TREC/KREC assay can be used as marker of thymic and bone marrow output.
