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Spedali Civili di Brescia

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR

Alessandra Sottini¹, Federico Serana¹, Diego Bertoli¹, Marco Chiarini¹, Monica Valotti¹, Marion Vaglio Tessitore¹, Luisa Imberti¹

¹CREA, Diagnostics Department, Spedali Civili di Brescia

Here, we describe a method for simultaneous quantification of T-cell receptor excision circles (TRECs) and K-deleting recombination excision circles (KRECs). The TREC/KREC assay can be used as marker of thymic and bone marrow output.