Goethe University of Frankfurt
2 ARTICLES PUBLISHED IN JoVE
Medicine
Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
Hanibal Bohnenberger 1, Philipp Ströbel 1, Sebastian Mohr 2, Jasmin Corso 3, Tobias Berg 2, Henning Urlaub 3,4, Christof Lenz 3,4, Hubert Serve 2,5,6, Thomas Oellerich 2,5,6
1Institute of Pathology, University Medical Center, Göttingen, 2Department of Hematology/Oncology, Goethe University of Frankfurt, 3Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, 4Bioanalytics, Institute of Clinical Chemistry, University Medical Center, Göttingen, 5German Cancer Consortium, 6German Cancer Research Center
In-depth analyses of cancer cell proteomes facilitate identification of novel drug targets and diagnostic biomarkers. We describe an experimental workflow for quantitative analysis of (phospho-)proteomes in cancer cell subpopulations derived from liquid and solid tumors. This is achieved by combining cellular enrichment strategies with quantitative Super-SILAC-based mass spectrometry.
Neuroscience
Custom-made Microdialysis Probe Design
Jana Lietsche 1, Jan Gorka 2, Stefanie Hardt 3, Michael Karas 2, Jochen Klein 1
1Department of Pharmacology, Goethe University of Frankfurt, 2Department of Pharmaceutical Chemistry, Goethe University of Frankfurt, 3Department of Clinical Pharmacology, Goethe University of Frankfurt
Microdialysis is a commonly used technique in neuroscience research. Therefore commercial probes are in great demand.In this work a probe assembly is explained in detail to build a reliable, concentric, custom-made microdialysis probe for less than $10.
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