Accedi

Goethe University of Frankfurt

2 ARTICLES PUBLISHED IN JoVE

Medicine

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Hanibal Bohnenberger¹, Philipp Ströbel¹, Sebastian Mohr², Jasmin Corso³, Tobias Berg², Henning Urlaub^3,4, Christof Lenz^3,4, Hubert Serve^2,5,6, Thomas Oellerich^2,5,6

¹Institute of Pathology, University Medical Center, Göttingen, ²Department of Hematology/Oncology, Goethe University of Frankfurt, ³Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, ⁴Bioanalytics, Institute of Clinical Chemistry, University Medical Center, Göttingen, ⁵German Cancer Consortium, ⁶German Cancer Research Center

In-depth analyses of cancer cell proteomes facilitate identification of novel drug targets and diagnostic biomarkers. We describe an experimental workflow for quantitative analysis of (phospho-)proteomes in cancer cell subpopulations derived from liquid and solid tumors. This is achieved by combining cellular enrichment strategies with quantitative Super-SILAC-based mass spectrometry.

Neuroscience

Custom-made Microdialysis Probe Design

Jana Lietsche¹, Jan Gorka², Stefanie Hardt³, Michael Karas², Jochen Klein¹

¹Department of Pharmacology, Goethe University of Frankfurt, ²Department of Pharmaceutical Chemistry, Goethe University of Frankfurt, ³Department of Clinical Pharmacology, Goethe University of Frankfurt

Microdialysis is a commonly used technique in neuroscience research. Therefore commercial probes are in great demand.In this work a probe assembly is explained in detail to build a reliable, concentric, custom-made microdialysis probe for less than $10.