Analysis of Translation Initiation During Stress Conditions by Polysome ProfilingLaëtitia Coudert *1,2, Pauline Adjibade *1,2, Rachid Mazroui 1,2
1Department of Molecular Biology, Medical Biochemistry, and Pathology, Faculty of Medicine, Laval University, 2CHU de Quebec Research Center
Here, we describe a method to analyze changes in the initiation of mRNA translation of eukaryotic cells in response to stress conditions. This method is based on the velocity separation on sucrose gradients of translating ribosomes from non-translating ribosomes.
