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CHU de Quebec Research Center

1 ARTICLES PUBLISHED IN JoVE

Biology

Analysis of Translation Initiation During Stress Conditions by Polysome Profiling

Laëtitia Coudert^*1,2, Pauline Adjibade^*1,2, Rachid Mazroui^1,2

¹Department of Molecular Biology, Medical Biochemistry, and Pathology, Faculty of Medicine, Laval University, ²CHU de Quebec Research Center

Here, we describe a method to analyze changes in the initiation of mRNA translation of eukaryotic cells in response to stress conditions. This method is based on the velocity separation on sucrose gradients of translating ribosomes from non-translating ribosomes.