The University of Tokyo Hospital
3 ARTICLES PUBLISHED IN JoVE
Medicine
Three-dimensional Co-culture Model for Tumor-stromal Interaction
Masafumi Horie 1,2, Akira Saito 1,3, Yoko Yamaguchi 4, Mitsuhiro Ohshima 5, Takahide Nagase 1
1Department of Respiratory Medicine, Graduate School of Medicine, The University of Tokyo, 2Department of Clinical Laboratory, Graduate School of Medicine, The University of Tokyo, 3Division for Health Service Promotion, The University of Tokyo, 4Department of Biochemistry, Nihon University School of Dentistry, 5Department of Biochemistry, Ohu University School of Pharmaceutical Sciences
Here we present a protocol to co-culture in three-dimensions, which is useful for investigating multicellular interactions and extracellular matrix-dependent modulation of cancer cell behavior. In this experimental model, cancer cells are cultured on collagen gels embedded with human cancer-associated fibroblasts.
Developmental Biology
Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition
Yu Mikami 1,2, Hirotaka Matsuzaki 2, Hideyuki Takeshima 2, Kosuke Makita 2, Yasuhiro Yamauchi 2, Takahide Nagase 2
1Department of Clinical Laboratory, The University of Tokyo Hospital, 2Department of Respiratory Medicine, The University of Tokyo Hospital
Here, we describe the development and application of a gel contraction assay for evaluating contractile function in mesenchymal cells that underwent epithelial-mesenchymal transition.
Biology
A Pacing-Controlled Procedure for the Assessment of Heart Rate-Dependent Diastolic Functions in Murine Heart Failure Models
Genri Numata 1,2, Eiki Takimoto 1,3
1Department of Cardiovascular Medicine, The University of Tokyo Hospital, 2Department of Therapeutic Strategy for Heart Failure, Graduate School of Medicine, The University of Tokyo, 3Division of Cardiology, Department of Medicine, The Johns Hopkins Medical Institutions
The present protocol describes obtaining the pressure-volume relationship through transesophageal pacing, which serves as a valuable tool in evaluating diastolic function in mouse models of heart failure.
