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Ross University School of Veterinary Medicine

1 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome
Ibrahim M. Shokry 1, John J. Callanan 1, John Sousa 2, Rui Tao 2
1Ross University School of Veterinary Medicine, 2Charles E. Schmidt College of Medicine, Florida Atlantic University

This protocol describes a rapid and simplified in situ hybridization method ideal forparaformaldehyde-prefixed brain, thus reducing the need for prolonged complex steps while using fresh frozen tissues. The method is validated using the identification of the serotonin 5-HT2A receptor gene htr2a in rats.

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