登录

Ross University School of Veterinary Medicine

1 ARTICLES PUBLISHED IN JoVE

image

Neuroscience

Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome
Ibrahim M. Shokry 1, John J. Callanan 1, John Sousa 2, Rui Tao 2
1Ross University School of Veterinary Medicine, 2Charles E. Schmidt College of Medicine, Florida Atlantic University

This protocol describes a rapid and simplified in situ hybridization method ideal forparaformaldehyde-prefixed brain, thus reducing the need for prolonged complex steps while using fresh frozen tissues. The method is validated using the identification of the serotonin 5-HT2A receptor gene htr2a in rats.

JoVE Logo

政策

使用条款

隐私

科研

教育

关于 JoVE

版权所属 © 2024 MyJoVE 公司版权所有,本公司不涉及任何医疗业务和医疗服务。