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Ross University School of Veterinary Medicine

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome

Ibrahim M. Shokry¹, John J. Callanan¹, John Sousa², Rui Tao²

¹Ross University School of Veterinary Medicine, ²Charles E. Schmidt College of Medicine, Florida Atlantic University

This protocol describes a rapid and simplified in situ hybridization method ideal forparaformaldehyde-prefixed brain, thus reducing the need for prolonged complex steps while using fresh frozen tissues. The method is validated using the identification of the serotonin 5-HT_2A receptor gene htr2a in rats.