Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber TechniqueWendy Hempstock 1, Noriko Ishizuka 1, Hisaysohi Hayashi 1
1Laboratory of Physiology, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka
Intestinal epithelium confers not only nutrient absorption but protection against noxious substances. The apical-most epithelial intercellular junction, i.e., the tight junction, regulates paracellular solute and ion permeability. Here, a protocol for the preparation of mucosal sheets and assessment of the ion selectivity of tight junctions using Ussing chamber technique is described.
