Plaquing of Herpes Simplex VirusesLauren A. Sadowski *1, Gregory M. Lesko *1, Chad Suissa *1, Rista Upadhyay *1,2, Prashant J. Desai 3, Barry J. Margulies 1,4
1Towson University Herpes Virus Lab, Department of Biological Sciences, Towson University, 2Towson University Department of Chemistry, Towson University, 3Department of Oncology, Johns Hopkins University School of Medicine, 4Molecular Biology, Biochemistry, and Bioinformatics Program, Towson University
Plaquing is a routine method used to quantify live viruses in a population. Though plaquing is frequently taught in various microbiology curricula with bacteria and bacteriophages, plaquing of mammalian viruses is more complex and time-consuming. This protocol describes the procedures that function reliably for regular work with herpes simplex viruses.
