Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered ProteinsClément Danis *1,2, Clément Despres *1, Luiza M. Bessa 1, Idir Malki 1, Hamida Merzougui 1, Isabelle Huvent 1, Haoling Qi 1, Guy Lippens 1, François-Xavier Cantrelle 1, Robert Schneider 1, Xavier Hanoulle 1, Caroline Smet-Nocca 1, Isabelle Landrieu 1
1UMR8576, CNRS, Lille University, 2UMR-S1172, INSERM CNRS, Lille University
We describe here a method to identify multiple phosphorylations of an intrinsically disordered protein by Nuclear Magnetic Resonance Spectroscopy (NMR), using Tau protein as a case study. Recombinant Tau is isotopically enriched and modified in vitro by a kinase prior to data acquisition and analysis.
