University of Kentucky Medical Center
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Using Enzyme-based Biosensors to Measure Tonic and Phasic Glutamate in Alzheimer's Mouse Models
Holly C. Hunsberger 1, Sharay E. Setti 2, Ryan T. Heslin 2, Jorge E. Quintero 3, Greg A. Gerhardt 3, Miranda N. Reed 2
1Department of Psychology, West Virginia University, 2Department of Drug Discovery & Development, Auburn University, 3Department of Neuroscience, University of Kentucky Medical Center
Here, we describe the setup, software navigation, and data analysis for a spatially and temporally precise method of measuring tonic and phasic extracellular glutamate changes in vivo using enzyme-linked microelectrode arrays (MEA).
Neuroscience
Adaptation of Microelectrode Array Technology for the Study of Anesthesia-induced Neurotoxicity in the Intact Piglet Brain
Emily D. Geyer *1, Prithvi A. Shetty *1, Christopher J. Suozzi *1, David Z. Allen *1,2, Pamela P. Benavidez *1,2, Joseph Liu *1,3, Charles N. Hollis 1, Greg A. Gerhardt 4, Jorge E. Quintero 4, Jason J. Burmeister 4, Emmett E. Whitaker 1,3
1Department of Anesthesiology, Ohio State University College of Medicine, 2Medical Student Research Program, Ohio State University College of Medicine, 3Department of Anesthesiology and Pain Medicine, Nationwide Children's Hospital, 4Department of Neuroscience, University of Kentucky Medical Center
This study explores the novel use of enzyme-based microelectrode array (MEA) technology to monitor in vivo neurotransmitter activity in piglets. The hypothesis was that glutamate dysregulation contributes to the mechanism of anesthetic neurotoxicity. Here, we present a protocol to adapt MEA technology to study the mechanism of anesthesia-induced neurotoxicity.
