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University of Kentucky Medical Center

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Using Enzyme-based Biosensors to Measure Tonic and Phasic Glutamate in Alzheimer's Mouse Models

Holly C. Hunsberger¹, Sharay E. Setti², Ryan T. Heslin², Jorge E. Quintero³, Greg A. Gerhardt³, Miranda N. Reed²

¹Department of Psychology, West Virginia University, ²Department of Drug Discovery & Development, Auburn University, ³Department of Neuroscience, University of Kentucky Medical Center

Here, we describe the setup, software navigation, and data analysis for a spatially and temporally precise method of measuring tonic and phasic extracellular glutamate changes in vivo using enzyme-linked microelectrode arrays (MEA).

Neuroscience

Adaptation of Microelectrode Array Technology for the Study of Anesthesia-induced Neurotoxicity in the Intact Piglet Brain

Emily D. Geyer^*1, Prithvi A. Shetty^*1, Christopher J. Suozzi^*1, David Z. Allen^*1,2, Pamela P. Benavidez^*1,2, Joseph Liu^*1,3, Charles N. Hollis¹, Greg A. Gerhardt⁴, Jorge E. Quintero⁴, Jason J. Burmeister⁴, Emmett E. Whitaker^1,3

¹Department of Anesthesiology, Ohio State University College of Medicine, ²Medical Student Research Program, Ohio State University College of Medicine, ³Department of Anesthesiology and Pain Medicine, Nationwide Children's Hospital, ⁴Department of Neuroscience, University of Kentucky Medical Center

This study explores the novel use of enzyme-based microelectrode array (MEA) technology to monitor in vivo neurotransmitter activity in piglets. The hypothesis was that glutamate dysregulation contributes to the mechanism of anesthetic neurotoxicity. Here, we present a protocol to adapt MEA technology to study the mechanism of anesthesia-induced neurotoxicity.