UNLP

1 ARTICLES PUBLISHED IN JoVE

Cancer Research

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures

Felipe J. Núñez^1,2, Flor M. Mendez², Maria B. Garcia-Fabiani^1,2, Joaquín Pardo^1,3, Marta Edwards^1,2, Pedro R. Lowenstein^1,2, Maria G. Castro^1,2

¹Department of Neurosurgery, University of Michigan Medical School, ²Department of Cell & Developmental Biology, University of Michigan, ³INIBIOLP, Histology B-Pathology B, School of Medicine, UNLP

Neurospheres grown as 3D cultures constitute a powerful tool to study glioma biology. Here we present a protocol to perform immunohistochemistry while maintaining the 3D structure of glioma neurospheres through paraffin embedding. This method enables the characterization of glioma neurosphere properties such as stemness and neural differentiation.