Center for Infectious Disease Research
2 ARTICLES PUBLISHED IN JoVE
Biology
Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae
Ramsey A. Saleem 1, John D. Aitchison 1
1Institute for Systems Biology
Description of a quantitative phosphorylation procedure using cryolysis, urea solubilziation, HILIC fractionation and IMAC enrichment of phosphorylated peptides.
Biology
ODELAY: A Large-scale Method for Multi-parameter Quantification of Yeast Growth
Thurston Herricks 1, Fred D. Mast 1,2, Song Li 1, John D. Aitchison 1,2
1Institute for Systems Biology, 2Center for Infectious Disease Research
We present a method for quantifying growth phenotypes of individual yeast cells as they grow into colonies on solid media using time-lapse microscopy termed, One-cell Doubling Evaluation of Living Arrays of Yeast (ODELAY). Population heterogeneity of genetically identical cells growing into colonies can be directly observed and quantified.
