European XFEL GmbH
2 ARTICLES PUBLISHED IN JoVE
Chemistry
An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers
Daniel Rolles 1, Rebecca Boll 2,3, Benjamin Erk 2, Dimitrios Rompotis 2, Bastian Manschwetus 2
1J.R. Macdonald Laboratory, Department of Physics, Kansas State University, 2Deutsches Elektronen-Synchrotron DESY, 3European XFEL GmbH
This protocol describes the key steps for performing and analyzing pump-probe experiments combining a femtosecond optical laser with a free-electron laser in order to study ultrafast photochemical reactions in gas-phase molecules.
Biochemistry
Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip
Raphaël de Wijn 1,4, Kévin Rollet 1,2, Vincent Olieric 3, Oliver Hennig 2, Nicola Thome 1, Camille Noûs 1, Caroline Paulus 1, Bernard Lorber 1, Heike Betat 2, Mario Mörl 2, Claude Sauter 1
1Université de Strasbourg, Architecture et Réactivité de l’ARN, UPR 9002, CNRS, Institut de Biologie Moléculaire et Cellulaire, 2Biochemistry and Molecular Biology, Institute for Biochemistry, Leipzig University, 3Paul Scherrer Institute, Swiss Light Source, 4European XFEL GmbH
A versatile microfluidic device is described that enables the crystallization of an enzyme using the counter-diffusion method, the introduction of a substrate in the crystals by soaking, and the 3D structure determination of the enzyme:substrate complex by a serial analysis of crystals inside the chip at room temperature.
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