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2 ARTICLES PUBLISHED IN JoVE

Chemistry

An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers

Daniel Rolles¹, Rebecca Boll^2,3, Benjamin Erk², Dimitrios Rompotis², Bastian Manschwetus²

¹J.R. Macdonald Laboratory, Department of Physics, Kansas State University, ²Deutsches Elektronen-Synchrotron DESY, ³European XFEL GmbH

This protocol describes the key steps for performing and analyzing pump-probe experiments combining a femtosecond optical laser with a free-electron laser in order to study ultrafast photochemical reactions in gas-phase molecules.

Biochemistry

Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip

Raphaël de Wijn^1,4, Kévin Rollet^1,2, Vincent Olieric³, Oliver Hennig², Nicola Thome¹, Camille Noûs¹, Caroline Paulus¹, Bernard Lorber¹, Heike Betat², Mario Mörl², Claude Sauter¹

¹Université de Strasbourg, Architecture et Réactivité de l’ARN, UPR 9002, CNRS, Institut de Biologie Moléculaire et Cellulaire, ²Biochemistry and Molecular Biology, Institute for Biochemistry, Leipzig University, ³Paul Scherrer Institute, Swiss Light Source, ⁴European XFEL GmbH

A versatile microfluidic device is described that enables the crystallization of an enzyme using the counter-diffusion method, the introduction of a substrate in the crystals by soaking, and the 3D structure determination of the enzyme:substrate complex by a serial analysis of crystals inside the chip at room temperature.