South Dakota State University

4 ARTICLES PUBLISHED IN JoVE

Environment

How to Create Conditioned Taste Aversion for Grazing Ground Covers in Woody Crops with Small Ruminants

Carmen L. Manuelian¹, Elena Albanell¹, Maristela Rovai^1,2, Gerardo Caja¹

¹Group of Ruminant Research (G2R), Department of Animal and Food Science, Universitat Autònoma de Barcelona, ²Dairy Science Department, South Dakota State University

Feeding behavior preferences in livestock can be modified to implement a grazing management plan in woody crops. Here, we present a protocol to show a lithium chloride dose after eating a new plant that induces conditioned taste aversion.

Education

Protocol for Assessing the Relative Effects of Environment and Genetics on Antler and Body Growth for a Long-lived Cervid

Eric S. Michel^1,2, Emily B. Flinn¹, Stephen Demarais¹, Bronson K. Strickland¹, Guiming Wang¹, Chad M. Dacus³

¹Department of Wildlife, Fisheries and Aquaculture, Mississippi State University, ²Department of Natural Resource Management, South Dakota State University, ³Mississippi Department of Wildlife, Fisheries and Parks

Phenotypic differences among cervid populations may be related to population-level genetics or nutrition; discerning which is difficult in the wild. This protocol describes how we designed a controlled study where nutritional variation was eliminated. We found that phenotypic variation of male white-tailed deer was more limited by nutrition than genetics.

Environment

In Situ Soil Moisture Sensors in Undisturbed Soils

Todd G. Caldwell¹, Michael H. Cosh², Steven R. Evett³, Nathan Edwards⁴, Heather Hofman⁵, Bradley G. Illston⁶, Tilden Meyers⁷, Marina Skumanich⁸, Kent Sutcliffe⁹

¹Nevada Water Science Center, U.S. Geological Survey, ²Agricultural Research Service, Hydrology and Remote Sensing Lab., U.S. Department of Agriculture, ³Agricultural Research Service, Conservation and Production Research Lab., U.S. Department of Agriculture, ⁴South Dakota State University, ⁵National Water and Climate Center, U.S. Department of Agriculture, ⁶University of Oklahoma, ⁷Air Resources Lab, National Oceanic and Atmospheric Administration, ⁸National Integrated Drought Information System, National Oceanic and Atmospheric Administration, ⁹Snow Survey Staff, U.S. Department of Agriculture

The determination of soil water content is a critical mission requirement for many state and federal agencies. This protocol synthesizes multi-agency efforts to measure soil water content using buried in situ sensors.

Cancer Research

Growing Desmoplastic Three-Dimensional Pancreatic Cancer Spheroids from Co-Culture

Rishabh Bahl¹, Cindy Venegas Mata¹, Benedikt Neth¹, Lara Meinzinger¹, Annika Celine Deppe¹, Haley Jahnke¹, Camryn Blackwell¹, Mikhail Durymanov¹, Joshua Reineke¹

¹Department of Pharmaceutical Sciences, South Dakota State University

Pancreatic cancer remains one of the toughest cancers to treat. Therefore, it is critical that pre-clinical models evaluating treatment efficacy are reproducible and clinically relevant. This protocol describes a simple co-culture procedure to generate reproducible, clinically relevant desmoplastic spheroids.