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2 ARTICLES PUBLISHED IN JoVE
JoVE Journal
The Multi-organ Chip - A Microfluidic Platform for Long-term Multi-tissue Coculture
Eva-Maria Materne *1, Ilka Maschmeyer *1,2, Alexandra K. Lorenz 1, Reyk Horland 1,2, Katharina M. S. Schimek 1, Mathias Busek 3, Frank Sonntag 3, Roland Lauster 1, Uwe Marx 1,2
1Medical Biotechnology, Technische Universität Berlin, 2TissUse GmbH, 3Fraunhofer IWS
Here, we present a protocol to coculture primary cells, tissue models and punch biopsies in a microfluidic multi-organ chip for up to 28 days. Human dermal microvascular endothelial cells, liver aggregates and skin biopsies were successfully combined in a common media circulation.
Bioengineering
A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates
Hao-Hsiang Hsu 1, John-Kevin Kracht 1, Laura Elisabeth Harder 1, Kerstin Rudnik 1, Gerd Lindner 2, Katharina Schimek 2, Uwe Marx 3, Ralf Pörtner 1
1Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology, 2Institute of Biotechnology, Department Medical Biotechnology, Technische Universität Berlin, 3TissUse GmbH
A method for determination of permeability in a membrane insert system for multi-well plates and in silico parameter optimization for the calculation of diffusion coefficients using simulation are presented.
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