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TissUse GmbH

2 ARTICLES PUBLISHED IN JoVE

JoVE Journal

The Multi-organ Chip - A Microfluidic Platform for Long-term Multi-tissue Coculture

Eva-Maria Materne^*1, Ilka Maschmeyer^*1,2, Alexandra K. Lorenz¹, Reyk Horland^1,2, Katharina M. S. Schimek¹, Mathias Busek³, Frank Sonntag³, Roland Lauster¹, Uwe Marx^1,2

¹Medical Biotechnology, Technische Universität Berlin, ²TissUse GmbH, ³Fraunhofer IWS

Here, we present a protocol to coculture primary cells, tissue models and punch biopsies in a microfluidic multi-organ chip for up to 28 days. Human dermal microvascular endothelial cells, liver aggregates and skin biopsies were successfully combined in a common media circulation.

Bioengineering

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates

Hao-Hsiang Hsu¹, John-Kevin Kracht¹, Laura Elisabeth Harder¹, Kerstin Rudnik¹, Gerd Lindner², Katharina Schimek², Uwe Marx³, Ralf Pörtner¹

¹Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology, ²Institute of Biotechnology, Department Medical Biotechnology, Technische Universität Berlin, ³TissUse GmbH

A method for determination of permeability in a membrane insert system for multi-well plates and in silico parameter optimization for the calculation of diffusion coefficients using simulation are presented.