National Agriculture and Food Research Organization
2 ARTICLES PUBLISHED IN JoVE
Environment
Tracking Infiltration Front Depth Using Time-lapse Multi-offset Gathers Collected with Array Antenna Ground Penetrating Radar
Hirotaka Saito 1, Seiichiro Kuroda 2, Toshiki Iwasaki 1, Haruyuki Fujimaki 3, Nobuhito Nagai 4, Jacopo Sala 5
1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 2National Institute for Rural Engineering, National Agriculture and Food Research Organization, 3Arid Land Research Center, Tottori University, 4GeoFive Co. Ltd, 53D-Radar
Here we present a Ground Penetrating Radar (GPR) system based on a ground-coupled, densely populated antenna array for monitoring the dynamic process of subsurface water infiltration. A time-lapse radar image of the infiltration process allowed estimating the depth of the wetting front during the course of the infiltration process.
Genetics
Primordial Germ Cell Cryopreservation and Revival of Drosophila Strains
Kaori Nishimura *1, Miho Asaoka *2, Yurina Sakamaki *2, Tatsuya Fukumoto *3,4, Daisuke Tanaka 3, Satoru Kobayashi 2, Toshiyuki Takano-Shimizu-Kouno 1
1KYOTO Drosophila Stock Center, Kyoto Institute of Technology, 2Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, 3Research Center of Genetic Resources, National Agriculture and Food Research Organization, 4Shizuoka Prefectural Ogasa High School
A long-term preservation method for Drosophila strains as an alternative to the frequent transfer of adult flies to fresh food vials is highly desirable. This protocol describes the cryopreservation of Drosophila primordial germ cells and strain revival via their transplantation to agametic host embryos.
