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National Agriculture and Food Research Organization

2 ARTICLES PUBLISHED IN JoVE

Environment

Tracking Infiltration Front Depth Using Time-lapse Multi-offset Gathers Collected with Array Antenna Ground Penetrating Radar

Hirotaka Saito¹, Seiichiro Kuroda², Toshiki Iwasaki¹, Haruyuki Fujimaki³, Nobuhito Nagai⁴, Jacopo Sala⁵

¹Graduate School of Agriculture, Tokyo University of Agriculture and Technology, ²National Institute for Rural Engineering, National Agriculture and Food Research Organization, ³Arid Land Research Center, Tottori University, ⁴GeoFive Co. Ltd, ⁵3D-Radar

Here we present a Ground Penetrating Radar (GPR) system based on a ground-coupled, densely populated antenna array for monitoring the dynamic process of subsurface water infiltration. A time-lapse radar image of the infiltration process allowed estimating the depth of the wetting front during the course of the infiltration process.

Genetics

Primordial Germ Cell Cryopreservation and Revival of Drosophila Strains

Kaori Nishimura^*1, Miho Asaoka^*2, Yurina Sakamaki^*2, Tatsuya Fukumoto^*3,4, Daisuke Tanaka³, Satoru Kobayashi², Toshiyuki Takano-Shimizu-Kouno¹

¹KYOTO Drosophila Stock Center, Kyoto Institute of Technology, ²Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, ³Research Center of Genetic Resources, National Agriculture and Food Research Organization, ⁴Shizuoka Prefectural Ogasa High School

A long-term preservation method for Drosophila strains as an alternative to the frequent transfer of adult flies to fresh food vials is highly desirable. This protocol describes the cryopreservation of Drosophila primordial germ cells and strain revival via their transplantation to agametic host embryos.