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Independent Contractor

2 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Method for High Speed Stretch Injury of Human Induced Pluripotent Stem Cell-derived Neurons in a 96-well Format
Jack K. Phillips 1, Sydney A. Sherman 1,2, Sevan R. Oungoulian 3, John D. Finan 1
1Department of Neurosurgery, NorthShore University HealthSystem, 2Midwestern University/Chicago College of Osteopathic Medicine, 3Independent Contractor

Here we present a method for a human in vitro model of stretch injury in a 96-well format on a timescale relevant to impact trauma. This includes methods for fabricating stretchable plates, quantifying the mechanical insult, culturing and injuring cells, imaging, and high content analysis to quantify injury.

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Education

A Friction Testing-Bioreactor Device for Study of Synovial Joint Biomechanics, Mechanobiology, and Physical Regulation
Lianna R. Gangi *1, Courtney A. Petersen *2, Sevan R. Oungoulian 2, Eben G. Estell 1, Krista M. Durney 1, Jason T. Suh 1, Gerard A. Ateshian 1,2, Clark T. Hung 1,3
1Department of Biomedical Engineering, Columbia University, 2Department of Mechanical Engineering, Columbia University, 3Department of Orthopedic Surgery, Columbia University

The present protocol describes a friction testing device that applies simultaneous reciprocal sliding and normal load to two contacting biological counterfaces.

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