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Independent Contractor

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Method for High Speed Stretch Injury of Human Induced Pluripotent Stem Cell-derived Neurons in a 96-well Format

Jack K. Phillips¹, Sydney A. Sherman^1,2, Sevan R. Oungoulian³, John D. Finan¹

¹Department of Neurosurgery, NorthShore University HealthSystem, ²Midwestern University/Chicago College of Osteopathic Medicine, ³Independent Contractor

Here we present a method for a human in vitro model of stretch injury in a 96-well format on a timescale relevant to impact trauma. This includes methods for fabricating stretchable plates, quantifying the mechanical insult, culturing and injuring cells, imaging, and high content analysis to quantify injury.

Education

A Friction Testing-Bioreactor Device for Study of Synovial Joint Biomechanics, Mechanobiology, and Physical Regulation

Lianna R. Gangi^*1, Courtney A. Petersen^*2, Sevan R. Oungoulian², Eben G. Estell¹, Krista M. Durney¹, Jason T. Suh¹, Gerard A. Ateshian^1,2, Clark T. Hung^1,3

¹Department of Biomedical Engineering, Columbia University, ²Department of Mechanical Engineering, Columbia University, ³Department of Orthopedic Surgery, Columbia University

The present protocol describes a friction testing device that applies simultaneous reciprocal sliding and normal load to two contacting biological counterfaces.