Hvidovre Hospital
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo
Line L. Holm 1,2,3, Milica Vukmanovic-Stejic 4, Thomas Blauenfeldt 1, Thomas Benfield 2,3, Peter Andersen 1, Arne N. Akbar 4, Morten Ruhwald 1
1Department of Infectious Disease Immunology, Center for Vaccine Research, Statens Serum Institut, 2Department of Infectious Diseases, Hvidovre Hospital, 3Institute of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, 4Division of Infection and Immunity, University College London
Here, we provide a demonstration of the suction blister cutaneous recall model. The model allows a simple access to study human in vivo adaptive immune responses, for instance in the context of vaccine development.
Medicine
Dual Test Gas Pulmonary Diffusing Capacity Measurement During Exercise in Humans Using the Single-Breath Method
Stine B. Nymand 1, Jacob Peter Hartmann 1,2,3, Helene Louise Hartmeyer 1, Iben E. Rasmussen 1, Amalie Bach Andersen 1, Milan Mohammad 1, Susan Al-Atabi 3, Birgitte Hanel 3, Ulrik Winning Iepsen 1,4, Jann Mortensen 3,5, Ronan M. G. Berg 1,2,3,6
1Centre for Physical Activity Research, Copenhagen University Hospital - Rigshospitalet, 2Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 3Department of Clinical Physiology and Nuclear Medicine, Copenhagen University Hospital - Rigshospitalet, 4Department of Anesthesiology and Intensive Care, Hvidovre Hospital, 5Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, 6Neurovascular Research Laboratory, Faculty of Life Sciences and Education, University of South Wales
This protocol presents a method to assess pulmonary alveolar-capillary reserve measured by combined single-breath measurement of the diffusing capacity to carbon monoxide (DL,CO) and nitric oxide (DL,NO) during exercise. Assumptions and recommendations for using the technique during exercise form the foundation of this article.
