Hvidovre Hospital

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo

Line L. Holm^1,2,3, Milica Vukmanovic-Stejic⁴, Thomas Blauenfeldt¹, Thomas Benfield^2,3, Peter Andersen¹, Arne N. Akbar⁴, Morten Ruhwald¹

¹Department of Infectious Disease Immunology, Center for Vaccine Research, Statens Serum Institut, ²Department of Infectious Diseases, Hvidovre Hospital, ³Institute of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, ⁴Division of Infection and Immunity, University College London

Here, we provide a demonstration of the suction blister cutaneous recall model. The model allows a simple access to study human in vivo adaptive immune responses, for instance in the context of vaccine development.

Medicine

Dual Test Gas Pulmonary Diffusing Capacity Measurement During Exercise in Humans Using the Single-Breath Method

Stine B. Nymand¹, Jacob Peter Hartmann^1,2,3, Helene Louise Hartmeyer¹, Iben E. Rasmussen¹, Amalie Bach Andersen¹, Milan Mohammad¹, Susan Al-Atabi³, Birgitte Hanel³, Ulrik Winning Iepsen^1,4, Jann Mortensen^3,5, Ronan M. G. Berg^1,2,3,6

¹Centre for Physical Activity Research, Copenhagen University Hospital - Rigshospitalet, ²Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, ³Department of Clinical Physiology and Nuclear Medicine, Copenhagen University Hospital - Rigshospitalet, ⁴Department of Anesthesiology and Intensive Care, Hvidovre Hospital, ⁵Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, ⁶Neurovascular Research Laboratory, Faculty of Life Sciences and Education, University of South Wales

This protocol presents a method to assess pulmonary alveolar-capillary reserve measured by combined single-breath measurement of the diffusing capacity to carbon monoxide (D_L,CO) and nitric oxide (D_L,NO) during exercise. Assumptions and recommendations for using the technique during exercise form the foundation of this article.