Qiqihar Medical University

1 ARTICLES PUBLISHED IN JoVE

Biology

Analysis of Nonhomologous End Joining and Homologous Recombination Efficiency in HEK-293T Cells Using GFP-Based Reporter Systems

Lu-Ping Zhang¹, Yong-Hong Nie¹, Tuo Tang¹, Ai-Xue Zheng¹, Xian Hong¹, Tao Wang¹

¹Laboratory of Protein Structure and Function, Institute of Medicine and Pharmacy, Qiqihar Medical University

This protocol describes an extrachromosomal nonhomologous end joining (NHEJ) assay and homologous recombination (HR) assay to quantify the efficiency of NHEJ and HR in HEK-293T cells.