Analysis of Nonhomologous End Joining and Homologous Recombination Efficiency in HEK-293T Cells Using GFP-Based Reporter SystemsLu-Ping Zhang 1, Yong-Hong Nie 1, Tuo Tang 1, Ai-Xue Zheng 1, Xian Hong 1, Tao Wang 1
1Laboratory of Protein Structure and Function, Institute of Medicine and Pharmacy, Qiqihar Medical University
This protocol describes an extrachromosomal nonhomologous end joining (NHEJ) assay and homologous recombination (HR) assay to quantify the efficiency of NHEJ and HR in HEK-293T cells.
